* 일시 : 11/21(목) 16:30 –
* 연사 : 이혜자 박사(국립보건연구원)
* 장소 : 벤처관 711호
* 제목 : 출생코호트 자료를 활용한 알레르기 질환발생과 환경요인 상관성 연구
*초록
Genetic variability is integrated with environmental exposures by epigenetic mechanisms. However, comprehensive study of epigenome-wide association has not been performed across childhood phenotypes of food allergy(FA). To discover epigenetic determinants for underlying mechanism of food allergy trajectory, we conducted the association study of epigenome-wide DNA methylation(Illumina HumanMethyl850K) in cord blood(n=371) in Cohort for Childhood Origins of Asthma and Allergic Diseases (COCOA). We used latent class trajectory analysis for phenotyping the natural course of FA from birth through 8 years-old(n=1,864). FA phenotypes were classified as 4 clusters: no FA(n=1,361, 87.3%), early remission(n=76, 6.9%), early persistent(n=62, 4.6%), and late remission(n=15, 1.1%). Early remission cluster of FA was associated with sensitization to milk at age 1 year and allergic rhinitis from at age 1 year to at age 5 years. Early persistent group showed the association with sensitization to milk and egg at age 1 year, sensitization to milk, egg, and Der-f at age 3 year, sensitization to Der-f at age 6 year, allergic rhinitis from at age 3 year to at age 5 years, and asthma from from at age 3 year to at age 7 years. Late remission cluster was associated with sensitization to egg at age 1 year, sensitization to egg and Der-f at age 3 year, and sensitization to egg and Der-f at age 6 year. All three clusters had an relation to atopic dermatitis from 1 years-old to 7 years-old. Also, we observed differential methylation profiles across multiple genes of two cluster phenotypes including genes in early remission(CAPZB, COL6A3, DLG2, KCNJ5, PDE11A, etc) and early persistent(BIRC3, CAMK4, CPT1B, PPP2R5E, SNTB1, etc) by pathway analysis. Multiple methylation sites of the cluster of early remission were in the pathway including ECM-receptor interaction and PI3K-Akt signalling. T-cell receptor signalling pathway showed the association with early persistent phenotypes. Our findings suggest insights into epigenetic regulatory pathways and underlying mechanism as biomarkers of childhood FA.